APE1 antibody (70R-12483)

Affinity purified Rabbit polyclonal APE1 antibody

Synonyms Polyclonal APE1 antibody, Anti-APE1 antibody, APEX antibody, apurinic-apyrimidinic endonuclease 1 antibody, APE-1, deoxyribonuclease (apurinic or apyrimidinic) antibody, apurinic/apyrimidinic (abasic) endonuclease antibody, APE1 antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, AP lyase antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, APEN antibody, redox factor-1 antibody, APE 1, APE antibody, APE-1 antibody, protein REF-1 antibody, APE1, AP endonuclease class I antibody, REF1 antibody, HAP1 antibody, APEX1 antibody, APE 1 antibody, APX antibody
Cross Reactivity Human
Applications ICC, IF, WB
Immunogen APE1 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 1 and 45 of APE1

Images

Immunofluorescent staining using APE1 antibody (70R-12483)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification APE1 antibody was purified by Protein A chromatography
Molecular Weight 36 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 5.04 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using APE1 antibody (70R-12483)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using APE1 antibody (70R-12483)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A549) using a 10 % SDS PAGE gel and APE1 antibody at a dilution of 1:3000

Availability: In stock

Price: €395.09
Size: 100 ul
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