APRIL protein (30R-AA042)
Purified recombinant Human APRIL protein
Overview
Overview
| Synonyms | TNFSF13 protein, TRDL-1a protein, TRDL-1 alpha protein, A Proliferating-inducing Ligand protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
Specifications
| Residues | Region of APRIL protein corresponding to amino acids AVLTQKQKKQ HSVLHLVPIN ATSKDDSDVT EVMWQPALRR GRGLQAQGYG VRIQDAGVYL LYSQVLFQDV TFTMGQVVSR EGQGRQETLF RCIRSMPSHP DRAYNSCYSA GVFHLHQGDI LSVIIPRARA KLNLSPHGTF LGFVKL. |
|---|---|
| Expression System | Hi-5 cells |
| Grade & Purity | > 95% pure |
| Molecular Weight | 16.3 kDa |
| Form & Buffer | Supplied as a lyophilized powder from 10mM Sodium Phosphate, pH 7.5 + 500mM NaCl + 5% Trehalose. |
Usage & Assay Information
| Bioactivity | “Determined by its ability to induce NF-?B activation in HEK293-NF-?B reporter cells transfected withBCMA receptor.” |
|---|
Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
|---|---|
| Endotoxin Levels | Endotoxin level is < 0.1 ng/μg of protein (< 1 EU/μg). |
General Information
| Biological Significance | APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product