APRT protein (30R-1483)
Purified recombinant Human APRT protein
|Synonyms||AMP pyrophosphorylase protein, MGC125856 protein, Adenine phosphoribosyltransferase AMP diphosphorylase protein, DKFZp686D13177 protein, MGC129961 protein, Transphosphoribosidase., AMP protein, MGC125857 protein|
Coomassie Blue stained SDS-PAGE of APRT protein (30R-1483)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-180 amino acids: MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE|
|Grade & Purity||> 90% pure|
|Molecular Weight||19.6 kDa (180aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E. coli and purified by using conventional chromatography techniques.|
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