APRT protein (30R-1483)

Purified recombinant Human APRT protein

Synonyms AMP pyrophosphorylase protein, MGC125856 protein, Adenine phosphoribosyltransferase AMP diphosphorylase protein, DKFZp686D13177 protein, MGC129961 protein, Transphosphoribosidase., AMP protein, MGC125857 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of APRT protein (30R-1483)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-180 amino acids: MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 19.6 kDa (180aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of APRT protein (30R-1483) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €206.99
Size: 100 ug
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