ARF5 protein (His tag) (80R-1859)
Purified recombinant ARF5 protein
|Synonyms||ARF-5, ARF 5 protein, ARF 5, ARF-5 protein, ARF5, ARF 5 protein, ADP ribosylation factor 5 protein, ADP-ribosylation factor 5 protein|
Coomassie Blue stained SDS-PAGE of ARF5 protein (His tag) (80R-1859)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-180 amino acids: MGSSHHHHHH SSGLVPRGSH MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR|
|Grade & Purity||> 90% pure|
|Molecular Weight||22.6 kDa (200aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ADP-ribosylation factor 5, also known as ARF5, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation. Recombinant human ARF5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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