ARF6 protein (His tag) (80R-1563)
Purified recombinant Human ARF6 protein
|Synonyms||ARF 6, ADP-ribosylation factor 6 protein, ARF 6 protein, ARF6, ARF-6 protein, ARF-6|
Coomassie Blue stained SDS-PAGE of ARF6 protein (His tag) (80R-1563)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-175 amino acids: MGSSHHHHHH SSGLVPRGSH MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGD GLYEGLTWLT SNYKS|
|Grade & Purity||> 95% pure|
|Molecular Weight||22.2 kDa (195aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 2mM DTT, 20% glycerol and 0.2mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. It is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. Additionally, ARF6 play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. Recombinant human ARF6 protein , fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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