ARID1A antibody (70R-51743)

Purified Polyclonal ARID1A antibody

Synonyms Polyclonal ARID1A antibody, Anti-ARID1A antibody, BAF250B antibody, DAN15 antibody, KIAA1235 antibody, OSA2 antibody, AT-rich interactive domain-containing protein 1B antibody, ARID domain-containing protein 1B antibody, BRG1-associated factor 250b antibody, BAF250B antibody, BRG1-binding protein hELD/OSA1 antibody, Osa homolog 2 antibody, hOsa2 antibody, p250R antibody
Specificity Recognizes endogenous levels of BAF250B protein
Cross Reactivity Mouse, Rat, Pig
Applications ICC, IF, IHC, WB
Immunogen ARID1A antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAF250B as the immunogen

Images

Western blot analysis using ARID1A antibody (70R-51743)

Western blot analysis of BAF250B expression in HeLa (A); HepG2 (B) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ARID1A antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ARID1A is a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin.

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Images

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Western blot analysis using ARID1A antibody (70R-51743)



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Western blot analysis of BAF250B expression in HeLa (A); HepG2 (B) whole cell lysates.
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Immunohistochemical analysis using ARID1A antibody (70R-51743)



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Immunohistochemical analysis of BAF250B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using ARID1A antibody (70R-51743)



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Immunofluorescent analysis of BAF250B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €161.96
Size: 100 ul
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