ARPC5 protein (His tag) (80R-1831)
Purified recombinant ARPC5 protein
|Synonyms||ARPC5, p16-ARC protein, ARPC-5, Actin related protein 2/3 complex protein, ARPC 5 protein, subunit 5 protein, ARPC-5 protein, ARPC 5, ARC16 protein|
Coomassie Blue stained SDS-PAGE of ARPC5 protein (His tag) (80R-1831)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-151 amino acids: MGSSHHHHHH SSGLVPRGSH MSKNTVSSAR FRKVDVDEYD ENKFVDEEDG GDGQAGPDEG EVDSCLRQGN MTAALQAALK NPPINTKSQA VKDRAGSIVL KVLISFKAND IEKAVQSLDK NGVDLLMKYI YKGFESPSDN SSAMLLQWHE KALAAGGVGS IVRVLTARKT V|
|Grade & Purity||> 90% pure|
|Molecular Weight||18.4 kDa (171aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 50% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||p16-ARC, also known as ARPC5, is a 151 amino acid subunit of the Arp2/3 complex. Thought to play a role in maintaining the integrity of Arp2/3, ARPC5 is a substrate for MAPKAPK-2 which, through phosphorylation of ARPC5, may participate in Arp2/3 regulatory functions and remodeling of the Actin cytoskeleton. Two isoforms of ARPC5 exist due to alternative splicing events. Recombinant human ARPC5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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