ASF1A protein (His tag) (80R-1135)

Purified recombinant Human ASF1A protein

Synonyms ASF1 anti-silencing function 1 homolog A Anti silencing function 1A protein, hAsf1a protein, HSPC146 protein, , ASF1 anti-silencing function 1 homolog A protein, ASFA-1, hCIA protein, CGI-98 protein, ASFA 1 protein, CIA protein, ASF1A, ASFA-1 protein, CGI 98 protein, CCG1 interacting factor A protein, Histone chaperone ASF1A protein, ASF1 anti silencing function 1 homolog A protein, ASFA 1, Histone chaperone ASF1A protein, CCG1-interacting factor A (CIA) protein, hAsf1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of ASF1A protein (His tag) (80R-1135)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-204 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAKV QVNNVVVLDN PSPFYNPFQF EITFECIEDL SEDLEWKIIY VGSAESEEYD QVLDSVLVGP VPAGRHMFVF QADAPNPGLI PDADAVGVTV VLITCTYRGQ EFIRVGYYVN NEYTETELRE NPPVKPDFSK LQRNILASNP RVTRFHINWE DNTEKLEDAE SSNPNLQSLL STDALPSASK GWSTSENSLN VMLESHMDCM
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 27 kDa (240aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance ASF1 anti-silencing function 1 homolog A, also known as ASF1A, belongs to H3/H4 family of histone chaperone proteins. ASF1A protein interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. Deletion of ASF1A in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint. Recombinant human ASF1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of ASF1A protein (His tag) (80R-1135) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €319.89
Size: 100 ug
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