ASPH protein (His tag) (80R-1260)

Purified recombinant Human ASPH protein

Synonyms HAAH protein, BAH protein, AAH protein, junctin protein, Aspartyl/asparaginyl beta-hydroxylase ASP beta hydroxylase protein, Peptide aspartate beta dioxygenase protein, , Aspartyl/asparaginyl beta hydroxylase protein, CASQ2BP1 protein, JCTN protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of ASPH protein (His tag) (80R-1260)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 75-270 amino acids: MGSSHHHHHH SSGLVPRGSH MFDLVDYEEV LGKLGIYDAD GDGDFDVDDA KVLLGLKERS TSEPAVPPEE AEPHTEPEEQ VPVEAEPQNI EDEAKEQIQS LLHEMVHAEH ETEHSYHVEE TVSQDCNQDM EEMMSEQENP DSSEPVVEDE RLHHDTDDVT YQVYEEQAVY EPLENEGIEI TEVTAPPEDN PVEDSQVIVE EVSIFPVEEQ QEVPPDT
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 24.5 kDa (217aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance ASPH (Aspartate beta-hydroxylase) is a widely-expressed type II membrane protein involved in calcium homeostasis. Located in the endoplasmic reticulum, ASPH specifically hydroxylates an Asp or Asn residue in the epidermal growth factor-like (EGF) domains of several proteins, using iron as a cofactor. While all ASPH variants are expressed in skeletal muscle, only some are detected in heart, brain, pancreas, placenta, lung, liver, and kidney tissues. Recombinant human ASPH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of ASPH protein (His tag) (80R-1260) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €319.89
Size: 100 ug
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