ASS1 antibody (70R-12570)

Affinity purified Rabbit polyclonal ASS1 antibody

Synonyms Polyclonal ASS1 antibody, Anti-ASS1 antibody, argininosuccinate synthetase 1 antibody, argininosuccinate synthase 1 antibody, ASS antibody, ASS1, ASS 1, ASS-1 antibody, ASS 1 antibody, ASS1 antibody, citrulline-aspartate ligase antibody, CTLN1 antibody, citrulline--aspartate ligase antibody, ASS-1, argininosuccinate synthase antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen ASS1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 155 and 412 of ASS1

Images

Immunofluorescent staining using ASS1 antibody (70R-12570)

Sample: HeLa cells were fixed in -20 deg C 100% MeOH for 5 min. Green: ASS1 protein stained by ASS1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Specifications

Host Rabbit
Isotype IgG
Method of Purification ASS1 antibody was purified by antigen-affinity chromatography
Molecular Weight 47 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.67 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using ASS1 antibody (70R-12570)</font></div>     | Sample: HeLa cells were fixed in -20 deg C 100% MeOH for 5 min. Green: ASS1 protein stained by ASS1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
  • <div><font face=Calibri size=2>Immunohistochemical staining using ASS1 antibody (70R-12570)</font></div>     | Immunohistochemical staining of paraffin-embedded mixed OVCA using ASS1 antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using ASS1 antibody (70R-12570)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:A431) using a 12 % SDS PAGE gel and ASS1 antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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