ATRIP antibody (70R-51765)

Purified Polyclonal ATRIP antibody

Synonyms Polyclonal ATRIP antibody, Anti-ATRIP antibody, AGS1 antibody, ATR-interacting protein antibody, ATM and Rad3-related-interacting protein antibody
Specificity Recognizes endogenous levels of ATRIP protein
Cross Reactivity Mouse, Rat
Applications FC, ICC, IF, IHC, WB
Immunogen ATRIP antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATRIP as the immunogen

Images

Western blot analysis using ATRIP antibody (70R-51765)

Western blot analysis of ATRIP expression in MCF7 (A); HeLa (B); NIH3T3 (C); rat liver (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification ATRIP antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, FC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance ATRIP is required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

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Images

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Western blot analysis using ATRIP antibody (70R-51765)



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Western blot analysis of ATRIP expression in MCF7 (A); HeLa (B); NIH3T3 (C); rat liver (D) whole cell lysates.
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Immunohistochemical analysis using ATRIP antibody (70R-51765)



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Immunohistochemical analysis of ATRIP staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using ATRIP antibody (70R-51765)



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Immunofluorescent analysis of ATRIP staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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