Avidin antibody (biotin) (60C-CF0103)

Rabbit polyclonal Avidin antibody (biotin) conjugated

Synonyms Polyclonal Avidin antibody, Anti-Avidin antibody
Specificity Chicken avidin
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Avidin antibody (biotin) was raised in rabbit using avidin isolated from hen egg white as the immunogen.

Specifications

Host Rabbit
Method of Purification Avidin antibody (biotin) was purified by ion exchange chromatography followed by dialysis.
Tag/Conjugate Biotin
Form & Buffer Supplied as a liquid in 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, 10 mg/ml BSA (IgG and Protease free), with 0.01% NaN3 as a preservative.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C or aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.

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Availability: In stock

Price: €305.78
Size: 1 ml
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