Avidin antibody (biotin) was purified by ion exchange chromatography followed by dialysis.
Tag/Conjugate
Biotin
Form & Buffer
Supplied as a liquid in 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, 10 mg/ml BSA (IgG and Protease free), with 0.01% NaN3 as a preservative.
Store at 4 deg C or aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
General Information
Biological Significance
Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.