Avidin antibody (HRP) was purified by ion exchange chromatography followed by dialysis.
Tag/Conjugate
HRP
Form & Buffer
IgG fraction supplied as a liquid in 10mM Potassium Phosphate and 75mM NaCl, pH 7.2 with 5mg/ml BSA (IgG and Protease free) and 0.005% Gentamicin Sulfate
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
General Information
Biological Significance
Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.