Avidin antibody (HRP) (60C-CF0104)

Rabbit polyclonal Avidin antibody (HRP) conjugated

Synonyms Polyclonal Avidin antibody, Anti-Avidin antibody
Specificity Chicken avidin
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Avidin antibody (HRP) was raised in rabbit using avidin isolated from hen egg white as the immunogen.

Specifications

Host Rabbit
Method of Purification Avidin antibody (HRP) was purified by ion exchange chromatography followed by dialysis.
Tag/Conjugate HRP
Form & Buffer IgG fraction supplied as a liquid in 10mM Potassium Phosphate and 75mM NaCl, pH 7.2 with 5mg/ml BSA (IgG and Protease free) and 0.005% Gentamicin Sulfate

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

General Information

Biological Significance Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.

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Availability: In stock

Price: €4,050.14
Size: 20 mg
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