Avidin-HRP (85R-1009)

Avidin-HRP conjugate for use in immunoassays

Synonyms Avidin-Horseradish Peroxidase
Specificity Human
Species Chicken
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Specifications

Form & Buffer Supplied in liquid form in PBS/BSA with 0.2% ProClin 150.
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C.

General Information

Biological Significance The avidin horseradish peroxidase is commonly used as a second step for detection of biotinylated antibodies by ELISA and immunohistochemical staining. Avidin binds to biotin with high affinity. [Note: Sodium azide is an inhibitor of horseradish peroxidase enzymatic activity and should not be added to the AV-HRP diluent.]

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