Avidin protein (30C-CE0101A)
Purified Avidin protein from hen egg white
Overview
Overview
| Species | Chicken |
|---|---|
| Protein Type | Binding Protein |
| Applications | User optimized |
Images
SDS-PAGE analysis of Avidin protein (30C-CE0101A)
Lane 1: Avidin - reduced. Lane 2: Avidin - non-reduced. Load: 1.0 ug per lane. Predicted/Observed size: 16 kDa, 16 kDa for Avidin.
Specifications
| Source | Chicken egg white |
|---|---|
| Method of Purification | Avidin protein was chromatographically purified. |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamicin supfate. Immunoglobulin and Protease free. DO NOT add NaN3. |
Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity. |
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