Avidin protein (Texas Red) (65C-CE0112)
Purified Avidin protein (Texas Red) from hen egg white
Overview
Overview
| Species | Chicken |
|---|---|
| Protein Type | Binding Protein |
| Applications | ELISA, FC, IF |
Images
Western Blot analysis using Avidin protein (Texas Red) (65C-CE0112)
Lane 2: Biotin conj ugated Guinea Pig IgG. Load: 100 ng per lane. Secondary antibody: Avidin Texas Red (TM) Conj ugated at 1:1, 000 for 30 min at RT. Block: 1% BSA-TTBS 30 min RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.
Specifications
| Source | Hen egg white |
|---|---|
| Method of Purification | Avidin protein (Texas Red) was purified by ion exchange chromatography followed by dialysis. |
| Tag/Conjugate | Texas Red |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:50,000, IF: 1:1,000-1:5,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity. The dissociation constant of avidin is measured to be kDa ˜ 10-15 M, making it one of the strongest known non-covalent bonds. |
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