Monoclonal Bax antibody, Anti-Bax antibody, Bcl-2–associated X protein antibody
Specificity
Mouse, Rat, Monkey, Human
Applications
FC, Functional Assay, IHC, IP, WB
Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
Store at 2-8 deg C. Do not freeze. Protect from light.
General Information
Biological Significance
The 6A7 antibody reacts with mouse, rat, monkey and human Bax. The 6A7 antibody shows no cross-reaction with Bcl-2 or Bcl-xL proteins. Bcl-2, Bax, and Bcl-xL are members of a protein family that regulates apoptosis while several family members including Bcl-2 and Bcl-xL inhibit apoptosis, overexpression of Bax promotes programmed cell death. A human Bax protein peptide sequence, aa 12-24, was used as immunogen.