beta 2 Microglobulin antibody (HRP) (60R-MR009HRP)
Rabbit polyclonal beta 2 Microglobulin antibody (HRP) conjugated
Overview
Overview
| Synonyms | Polyclonal beta 2 Microglobulin antibody, Anti-beta 2 Microglobulin antibody, B2M antibody, beta Microglobulin-2, beta Microglobulin 2 antibody, beta 2 microglobulin precursor antibody, beta chain of mhc class 1 proteins antibody, beta 2 Microglobulin, beta Microglobulin-2 antibody, Hdcma22p antibody, beta Microglobulin 2 |
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| Specificity | Human beta-2-macrogloculin |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Beta-2 microglobulin antibody (HRP) was raised in rabbit using beta-2-microglobulin from human urine as the immunogen. |
Images
Immunohistochemical staining using beta 2 Microglobulin antibody (HRP) (60R-MR009HRP)
Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Left panel: isotype control, Right panel:beta 2 Microglobulin antibody at 1 ug/ml for 20 min at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta 2 Microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: beta 2 Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Beta 2 Microglobulin antibody (HRP) was purified by ion exchange chromatography followed by dialysis. |
| Tag/Conjugate | HRP |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA and 0.01% gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:2,000-1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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General Information
| Biological Significance | Beta2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the Beta2 microglobulin protein is encoded by the B2M gene. |
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