Recognizes endogenous levels of Beta-actin protein
Cross Reactivity
Mouse, Rat, Bovine, Chicken, Dog, Monkey, Pig, Rabbit, Sheep
Applications
IHC, WB
Immunogen
Beta actin antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Beta-actin as the immunogen
Western blot analysis using Beta actin antibody (70R-49328)
Western blot analysis of Beta-actin expression in Jurkat (A); HeLa (B); NIH3T3 (C); rat liver (D) whole cell lysates.
Beta actin antibody
Western blot analysis using Beta actin antibody (70R-49328)
Western blot analysis of Beta-actin expression in Jurkat (A); HeLa (B); NIH3T3 (C); rat liver (D) whole cell lysates.
Immunohistochemical analysis using Beta actin antibody (70R-49328)
Immunohistochemical analysis of Beta-actin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Specifications
Host
Rabbit
Method of Purification
Beta actin antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Beta actin is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.