beta Tubulin 3 antibody (70R-10639)
Affinity purified Chicken polyclonal Beta Tubulin 3 antibody
Overview
Overview
| Synonyms | Polyclonal Beta Tubulin 3 antibody, Anti-Beta Tubulin 3 antibody, Tubulin 3 antibody, Beta-Tubulin 3 antibody, TUJ antibody, B Tubulin antibody |
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| Applications | ICC, IHC, WB |
| Immunogen | Beta Tubulin 3 antibody was raised in Chicken using two synthetic peptide (Human NP_AAL28094 and Rat AAM28438) / keyhole limpet hemocyanin (KLH) conjugates corresponding to different regions of the Beta Tubulin 3 gene product as the immunogen. |
Images
Immunofluorescent staining of beta Tubulin 3 antibody (70R-10639)
Dissociated cell culture of a neonatal mouse brain showing beta Tubulin 3 (green staining) of neurons. These cultures were counter-stained with a rabbit anti-GFAP antibody (red staining) to identify astrocytes, and with DAPI (blue staining) to localize nuclei.
Specifications
| Host | Chicken |
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| Method of Purification | Beta Tubulin 3 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative. |
| Concentration | 100 ug/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:1000-1:2000; IHC: 1:1000-1:2000; WB: 1:2000-1:5000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | Tubulin is the major component of microtubules, essential cell cytoskeleton proteins involved in mitosis, cytokinesis and transport of vesicles and organelles. Tubulin is a dimer composed of one alpha and one beta tubulin molecule, which assemble as microtubules in a GTP-dependent manner. The beta-Tubulin molecule exerts GTPase activity to hydrolyze to GDP, effectively governing microtubule formation. |
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