beta Tubulin 3 antibody (70R-10639)

Affinity purified Chicken polyclonal Beta Tubulin 3 antibody

Synonyms Polyclonal Beta Tubulin 3 antibody, Anti-Beta Tubulin 3 antibody, Tubulin 3 antibody, Beta-Tubulin 3 antibody, TUJ antibody, B Tubulin antibody
Applications ICC, IHC, WB
Immunogen Beta Tubulin 3 antibody was raised in Chicken using two synthetic peptide (Human NP_AAL28094 and Rat AAM28438) / keyhole limpet hemocyanin (KLH) conjugates corresponding to different regions of the Beta Tubulin 3 gene product as the immunogen.

Images

Immunofluorescent staining of beta Tubulin 3 antibody (70R-10639)

Dissociated cell culture of a neonatal mouse brain showing beta Tubulin 3 (green staining) of neurons. These cultures were counter-stained with a rabbit anti-GFAP antibody (red staining) to identify astrocytes, and with DAPI (blue staining) to localize nuclei.

Specifications

Host Chicken
Method of Purification Beta Tubulin 3 antibody was purified by affinity chromatography.
Form & Buffer Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative.
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 1:1000-1:2000; IHC: 1:1000-1:2000; WB: 1:2000-1:5000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light.

General Information

Biological Significance Tubulin is the major component of microtubules, essential cell cytoskeleton proteins involved in mitosis, cytokinesis and transport of vesicles and organelles. Tubulin is a dimer composed of one alpha and one beta tubulin molecule, which assemble as microtubules in a GTP-dependent manner. The beta-Tubulin molecule exerts GTPase activity to hydrolyze to GDP, effectively governing microtubule formation.

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Images

  • Immunofluorescent staining of beta Tubulin 3 antibody (70R-10639) | Dissociated cell culture of a neonatal mouse brain showing beta Tubulin 3 (green staining) of neurons. These cultures were counter-stained with a rabbit anti-GFAP antibody (red staining) to identify astrocytes, and with DAPI (blue staining) to localize nuclei.

Availability: In stock

Price: €502.25
Size: 1 ml
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