beta Tubulin antibody (70R-12567)

Affinity purified Rabbit polyclonal beta Tubulin antibody

Synonyms Polyclonal beta Tubulin antibody, Anti-beta Tubulin antibody, TUBB antibody, MGC16435 antibody, TUBB5 antibody, beta1-tubulin antibody, MGC117247 antibody, class I beta-tubulin antibody, beta 5-tubulin antibody, OK/SW-cl.56 antibody, tubulin beta polypeptide antibody, tubulin beta chain antibody, tubulin beta-5 chain antibody, TUBB1 antibody, M40 antibody, tubulin beta polypeptide antibody, beta-4 tubulin antibody, beta Ib tubulin antibody, tubulin beta-1 chain antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen beta Tubulin antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 238 and 429 of beta Tubulin

Images

Western blot analysis using beta Tubulin antibody (70R-12567)

Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10 % SDS PAGE gel and beta Tubulin antibody at a dilution of 1:5000

Specifications

Host Rabbit
Isotype IgG
Method of Purification beta Tubulin antibody was purified by antigen-affinity chromatography
Molecular Weight 50 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, WB: 1:1000-1:10000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using beta Tubulin antibody (70R-12567)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3) using a 10 % SDS PAGE gel and beta Tubulin antibody at a dilution of 1:5000
  • <div><font face=Calibri size=2>Immunohistochemical staining using beta Tubulin antibody (70R-12567)</font></div>     | Immunohistochemical staining of paraffin-embedded AGS xenograft using beta Tubulin antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using beta Tubulin antibody (70R-12567)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: 293T; B: H1299) using a 10 % SDS PAGE gel and beta Tubulin antibody at a dilution of 1:3000
  • <div><font face=Calibri size=2>Immunofluorescent staining using beta Tubulin antibody (70R-12567)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

Availability: In stock

Price: €323.11
Size: 100 ul
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