Bid antibody (70R-11670)
Rabbit polyclonal Bid antibody
Overview
Overview
| Synonyms | Polyclonal Bid antibody, anti-Bid antibody, MGC15319 antibody, MGC42355 antibody, FP497 antibody |
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| Cross Reactivity | Human |
| Applications | IHC, IP, WB |
| Immunogen | Bid antibody was raised in Rabbit using synthetic peptide surrounding amino acid 37 of human Bid as the immunogen. |
| Assay Information | Bid Blocking Peptide, catalog no. 33R-10654, is also available for use as a blocking control in assays to test for specificity of this Bid antibody |
Images
Western Blot analysis using Bid antibody (70R-11670)
Western blot analysis of Bid expression in Jurkat cell lysate.
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | Bid antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | IP: 5-10 ug/ml, IHC: 20-40 ug/ml, WB: 0.5-4 ug/ml |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas. |
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