Bid antibody (70R-11670)

Rabbit polyclonal Bid antibody

Synonyms Polyclonal Bid antibody, anti-Bid antibody, MGC15319 antibody, MGC42355 antibody, FP497 antibody
Cross Reactivity Human
Applications IHC, IP, WB
Immunogen Bid antibody was raised in Rabbit using synthetic peptide surrounding amino acid 37 of human Bid as the immunogen.
Assay Information Bid Blocking Peptide, catalog no. 33R-10654, is also available for use as a blocking control in assays to test for specificity of this Bid antibody

Images

Western Blot analysis using Bid antibody (70R-11670)

Western blot analysis of Bid expression in Jurkat cell lysate.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Bid antibody was purified by affinity chromatography
Form & Buffer Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations IP: 5-10 ug/ml, IHC: 20-40 ug/ml, WB: 0.5-4 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

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Images

  • <div><font face=Calibri size=2>Western Blot analysis using Bid antibody (70R-11670)</font></div>     | Western blot analysis of Bid expression in Jurkat cell lysate.

Availability: In stock

Price: €282.21
Size: 100 ug
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