Biotin reagent (Alk Phos) (65C-CE0201)
Alkaline Phosphatase conjugated biotin labelling reagent
Overview
Overview
| Protein Type | Synthetic |
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| Applications | ELISA, IHC, WB |
Specifications
| Method of Purification | Biotin reagent (Alk Phos) was purified by ion exchange chromatography followed by dialysis. |
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| Tag/Conjugate | Alkaline Phosphatase |
| Form & Buffer | 0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500 |
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Storage & Safety
| Storage | Store at 4 deg C. DO NOT FREEZE. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis. |
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