Biotin reagent (Glucose Oxidase) (65C-CE0203)
Glucose Oxidase conjugated biotin labelling reagent
Dot Blot analysis using Biotin reagent (Glucose Oxidase) (65C-CE0203)
Lane 1: Biotin Conj ugated Glucose Oxidase. Lane 2: Glucose Oxidase. Load: 3-fold serial dilution starting at 200 ng. Secondary antibody: HRP Streptavidin at 1:40, 000 for 30 min at RT. Block: 1% BSA-TTBS 30 min at RT.
|Method of Purification||Biotin reagent (Glucose Oxidase) was purified by ion exchange chromatography followed by dialysis.|
|Form & Buffer||Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500|
Storage & Safety
|Storage||Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.|
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