Biotin reagent (Glucose Oxidase) (65C-CE0203)

Glucose Oxidase conjugated biotin labelling reagent

Protein Type Synthetic
Applications ELISA, IHC, WB

Images

Dot Blot analysis using Biotin reagent (Glucose Oxidase) (65C-CE0203)

Lane 1: Biotin Conj ugated Glucose Oxidase. Lane 2: Glucose Oxidase. Load: 3-fold serial dilution starting at 200 ng. Secondary antibody: HRP Streptavidin at 1:40, 000 for 30 min at RT. Block: 1% BSA-TTBS 30 min at RT.

Specifications

Method of Purification Biotin reagent (Glucose Oxidase) was purified by ion exchange chromatography followed by dialysis.
Tag/Conjugate Glucose Oxidase
Form & Buffer Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl.

Usage & Assay Information

Usage Recommendations ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Dot Blot analysis using Biotin reagent (Glucose Oxidase) (65C-CE0203) | Lane 1: Biotin Conj ugated Glucose Oxidase. Lane 2: Glucose Oxidase. Load: 3-fold serial dilution starting at 200 ng. Secondary antibody: HRP Streptavidin at 1:40, 000 for 30 min at RT. Block: 1% BSA-TTBS 30 min at RT.

Availability: In stock

Price: €164.65
Size: 5 mg
OR
Shipping
View Our Distributors