Biotin reagent (HRP) (65C-CE0202)
HRP conjugated biotin labelling reagent
Dot Blot analysis using Biotin reagent (HRP) (65C-CE0202)
Antigen: Streptavidin. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Biotin (HRP) at 1:1,000 for 1 HR at RT. Blocked for 1 HR at RT.
|Method of Purification||Biotin reagent (HRP) was purified by ion exchange chromatography followed by dialysis.|
|Form & Buffer||Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:20,000-1:200,000, IHC: 1:1,000-1:5,000, WB: 1:10,000-1:40,000|
Storage & Safety
|Storage||Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.|
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