BLK protein (His tag) (80R-1979)
Recombinant human BLK protein (His tag)
Overview
Overview
| Synonyms | MODY11 protein, MGC10442 protein, Tyrosine-protein kinase Blk protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of BLK protein (His tag) (80R-1979)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-505 amino acids: MGSSHHHHHH SSGLVPRGSH MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ YELQP |
|---|---|
| Expression System | Hi-5 cells |
| Grade & Purity | > 80% pure |
| Molecular Weight | 59.8 kDa (525aa) |
| Tag/Conjugate | His tag |
| Form & Buffer | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT,0.1mM PMSF |
| Concentration | 0.25 mg/ml |
Usage & Assay Information
| Usage Recommendations | BLK protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user. |
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Storage & Safety
| Storage | Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles. |
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General Information
| Biological Significance | BLK, also known as B lymphoid kinase, is a 55kDa tyrosine kinase with SH3, SH2, and catalytic domains that contain consensus sequences of the Src protein tyrosine kinase family. This protein is expressed specifically in the B cell lineage and plays a role in the signaltransduction pathway that is restricted to B lymphoid cells. Recombinant human BLK protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques. |
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