BMF protein (T7 tag) (80R-1154)
Purified recombinant Human BMF protein (T7 tag)
Overview
Overview
| Synonyms | Bcl2 modifying factor protein, FLJ00065., BMF protein, isoform 3 protein, Bcl2 modifying factor protein, isoform 3 Bcl 2 modifying factor protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of BMF protein (T7 tag) (80R-1154)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-129 amino acids: MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 15.6 kDa (144aa), confirmed by MALDI-TOF. |
| Tag/Conjugate | T7 tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, and 10% glycerol. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | < 1.0 EU per ug of protein (determined by LAL method) |
General Information
| Biological Significance | Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types. Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. |
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