BMP7 protein (30R-AB031)
RecombinantHuman BMP7 protein
Overview
Overview
| Synonyms | BMP 7 protein, BMP7, Bone Morphogenetic protein 7 protein, BMP-7, BMP-7 protein, OP-1 protein, BMP 7, BMP-7 protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
Specifications
| Residues | Region of BMP7 protein corresponding to amino acids MANVAENSSS DQRQACKKHE LYVSFRDLGW QDWIIAPEGY AAYYCEGECA FPLNSYMNAT NHAIVQTLVH FINPETVPKP CCAPTQLNAI SVLYFDDSSN VILKKYRNMV VRACGCH. |
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| Expression System | CHO cells |
| Grade & Purity | > 98% pure |
| Method of Purification | SDS-PAGE |
| Molecular Weight | 26.4 kDa |
| Form & Buffer | Supplied as a lyophilized powder. |
Usage & Assay Information
| Bioactivity | Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04 ug/ml. |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | Endotoxin level is less than 0.1 ng per ug (1 EU/ug). |
General Information
| Biological Significance | Recombinant Human BMP-7 is a homodimeric glycoprotein consisting of two 117 amino acid subunits,which correspond to amino acid residues 315 to 431 of the full-length BMP-7 precursor. RecombinantHuman BMP-7 has a calculated molecular weight of 26.4 kDa; however, due to glycosylation, the BMP-7homodimer migrates at an apparent molecular weight of approximately 25-35 kDa by SDS-PAGE analysisunder non-reducing conditions. |
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