BNIP2 antibody (70R-49423)

Purified Polyclonal BNIP2 antibody

Synonyms Polyclonal BNIP2 antibody, Anti-BNIP2 antibody, NIP2 antibody, BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 antibody
Specificity Recognizes endogenous levels of BNIP2 protein
Cross Reactivity Mouse, Rat
Applications FC, ICC, IF, WB
Immunogen BNIP2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BNIP2 as the immunogen

Images

Western blot analysis using BNIP2 antibody (70R-49423)

Western blot analysis of BNIP2 expression in HEK293T (A); mouse liver (B) whole cell lysates.

Specifications

Host Rabbit
Method of Purification BNIP2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, FC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance BNIP2 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death.

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Images

  • 

Western blot analysis using BNIP2 antibody (70R-49423)



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Western blot analysis of BNIP2 expression in HEK293T (A); mouse liver (B) whole cell lysates.
  • 

Immunofluorescent analysis using BNIP2 antibody (70R-49423)



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Immunofluorescent analysis of BNIP2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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