Bordetella pertussis IgG ELISA Kit (55R-RE56141)

ELISA kit for detection of Bordetella pertussis IgG in the research laboratory

Applications ELISA
Assay Information Diluted patient serumor standards are applied to the microtiter stripswhich are coated with Bordetella pertussis antigen. Binding between the IgG antibody(if present)within the serum and the Bordetella antigen takes place. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for hepatitis B surface antigen as well as HIV antibody. However, there is no test method that can offer complete assurance therefore all re

General Information

Biological Significance Whooping cough is a disease of the respiratory tracts which is caused by Bordetella pertussis bacteria. It is transmitted by airborne infection. The gramnegative Coccobacillus produces a series of biologically active molecules. The different compounds appear either during the pathogenesis or during the process of immunization against pertussis and show different effects. A characterisation has been made for the pertussis toxin (pt), the filamentery haemagglutinine (fha) and different lipopolysaccharides (lps).

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Bordetella pertussis IgG ELISA Kit Protocol

Added: Sep 29, 2015

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Availability: In stock

Price: €202.28
Size: 96 wells
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