Bovine Albumin antibody (20-BR67)
Rabbit polyclonal Bovine Albumin antibody
Overview
Overview
| Synonyms | Polyclonal Bovine Albumin antibody, Anti-Bovine Albumin antibody, DKFZp779N1935 antibody, BSA antibody, Allergen Bos d 6 antibody, ALB antibody, Albumin antibody |
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| Specificity | Bovine serum albumin |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Bovine albumin antibody was raised in rabbit using bovine serum albumin as the immunogen. |
Images
Western blot analysis using Bovine Albumin antibody (20-BR67)
Western Blot of Bovine Serum Albumin antibody. Lane 1: BSA. Lane 2: None. Load: 50 ng per lane. BSA antibody at 1:1,000 for overnight at 4 deg C. Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: 5% Blocking buffer-TBS for 30 min at RT. Size: 66 kDa, 66 kDa for BSA.
Specifications
| Host | Rabbit |
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| Form & Buffer | Delipidated and defibrinated serum. Buffer contains 0.1M Na3PO4, 0.15M NaCl, pH 7.5 and 0.02% NaN3. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Bovine serum albumin is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. It is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. |
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