BRAF protein (His tag) (80R-4059)
Recombinant Human BRAF protein (His tag)
Overview
Overview
| Synonyms | Serine/threonine-protein kinase B-raf protein, B-RAF1 protein, BRAF1 protein, NS7 protein, RAFB1 protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS-PAGE analysis of BRAF protein (His tag) (80R-4059)
Figure annotated with amount of protein analysed and % SDS PAGE gel.
Specifications
| Residues | 432-766 amino acids: MGSSHHHHHH SSGLVPRGSH MGSEFSEDRN RMKTLGRRDS SDDWEIPDGQ ITVGQRIGSG SFGTVYKGKW HGDVAVKMLN VTAPTPQQLQ AFKNEVGVLR KTRHVNILLF MGYSTKPQLA IVTQWCEGSS LYHHLHIIET KFEMIKLIDI ARQTAQGMDY LHAKSIIHRD LKSNNIFLHE DLTVKIGDFG LATVKSRWSG SHQFEQLSGS ILWMAPEVIR MQDKNPYSFQ SDVYAFGIVL YELMTGQLPY SNINNRDQII FMVGRGYLSP DLSKVRSNCP KAMKRLMAEC LKKKRDERPL FPQILASIEL LARSLPKIHR SASEPSLNRA GFQTEDFSLY ACASPKTPIQ AGGYGAFPVH |
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| Expression System | E.coli |
| Grade & Purity | > 80% pure |
| Molecular Weight | 40.6 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as liquid in 20mM Tris-HCl, pH 8.0 containing 10% glycerol |
| Concentration | 250 ug/ml |
Storage & Safety
| Storage | Store at 4 deg C short term. Aliquot and store at -70 deg C for long term and avoid repeated freeze thaw cycles |
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General Information
| Biological Significance | BRAF belongs to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. Recombinant human BRAF protein, fused to His-tag at N-terminus, was expressed in E.coli. |
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