BRF1 antibody (10R-1234)

Mouse monoclonal BRF1 antibody

Synonyms Monoclonal BRF1 antibody, Anti-BRF1 antibody, hBRF antibody, BRF-1 antibody, MGC105048 antibody, BRF-1, BRF 1, Transcription factor IIIB antibody, BRF 1 antibody, BRF antibody, GTF3B antibody, TF3B90 antibody, TAFIII90 antibody, TAF3B2 antibody, BRF1, TAF3C antibody, TFIIIB90 antibody
Specificity Human BRF1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen BRF1 antibody was raised in mouse using recombinant Brf1 Homolog, Subunit Of Rna Polymerase Iii Transcription Initiation Factor Iiib (S. Cerevisiae) (Brf1)

Images

Immunofluorescent staining using BRF1 antibody (10R-1234)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BRF1 antibody.

Specifications

Host Mouse
Clone BRF1G2A8
Isotype IgG1
Method of Purification BRF1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer BRF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Three alternatively spliced variants encoding three different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. A transcript encoding a fourth isoform has not yet been completely characterized.

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Images

  • Immunofluorescent staining using BRF1 antibody (10R-1234) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BRF1 antibody.
  • Western Blot analysis of immunized recombinant protein using BRF1 antibody (10R-1234)

Availability: In stock

Price: €364.83
Size: 100 ug
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