Western Blot analyisis using c-Jun antibody (20R-2188)
Western blot analysis of extracts from 3T3, HUVEC and 293 cells using c-Jun antibody.
c-Jun antibody
Western Blot analyisis using c-Jun antibody (20R-2188)
Western blot analysis of extracts from 3T3, HUVEC and 293 cells using c-Jun antibody.
Immunohistochemical staining using c-Jun antibody (20R-2188)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun antibody (left), or the same antibody preincubated with blocking peptide (right).
Specifications
Host
Rabbit
Method of Purification
c-Jun antibody was purified by immunoaffinity chromatography.
Form & Buffer
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
c-Jun is the name of a gene and protein that, in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function. c-Jun knockout is lethal, but transgenic animals with a mutated c-Jun that cannot be phosphorylated (termed c-JunAA) can survive.