CA13 protein (80R-4197)
Recombinant Human CA13 protein with His tag
Overview
Overview
| Synonyms | Human CA13 protein, Recombinant CA13 protein, Carbonic anhydrase 13 protein, CAXIII protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | Control, SDS-PAGE |
Images
Coomassie blue stained SDS-PAGE showing analysis of CA13 protein(80R-4197)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-262 amino acids of Human CA13 protein: MGSSHHHHHH SSGLVPRGSP MGSMSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH |
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| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 31.8 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as liquid in PBS (pH 7.4) containing 10% glycerol and 1mM DTT |
| Concentration | 500 ug/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles. |
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General Information
| Biological Significance | CA13 also known as carbonic anhydrase 13 belongs to the alpha-carbonic anhydrase family. The carbonic anhydrase from a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that occurs relatively slowly in the absence of catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are claasified as metalloenzymes. There are at least five distinct CA families (alpha, beta, gamma, delta, and epsilon). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The alpha-CAs are found in humans. Recombinant human CA13, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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