Calf Intestinal Alkaline Phosphatase antibody (20C-CR2110RP)
Rabbit polyclonal Alkaline Phosphatase antibody (Calf Intestinal)
Overview
Overview
| Synonyms | Polyclonal Alkaline Phosphatase antibody, rabbit anti-Alkaline Phosphatase Antibody, ALPI antibody, IAP antibody, Alkaline phosphomonoesterase antibody, Glycerophosphatase antibody, Kasahara isozyme antibody, Intestinal alkaline phosphatase antibody |
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| Specificity | Bovine alkaline phosphotase |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Alkaline phosphatase antibody was raised in rabbit using alkaline phosphatase isolated from calf intestine as the immunogen. |
Images
Western blot analysis using Calf Intestinal Alkaline Phosphatase antibody (200-4135)
Western Blot of rabbit anti Alkaline Phosphatase antibody Lane 1: Calf intestine Alkaline Phosphatase. Load: ~1.5 ug Block: 5% Blocking buffer 1 hour at 4 deg C. diluted to 1:1000 in 5% Blocking buffer overnight at 4 deg C. Goat anti Rabbit 611-103-122 Lot
Specifications
| Host | Rabbit |
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| Method of Purification | Calf Intestinal Alkaline Phosphatase antibody was purified by ion exchange chromatography followed by dialysis. |
| Form & Buffer | Supplied in lyophilized form in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution, aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. |
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