Calpain 5 antibody (70R-12717)

Affinity purified Rabbit polyclonal Calpain 5 antibody

Synonyms Polyclonal Calpain 5 antibody, Anti-Calpain 5 antibody, Calpain -5, FLJ46245 antibody, new calpain 3 antibody, nCL-3 antibody, calpain-5 antibody, Calpain 5 antibody, Calpain -5 antibody, CAPN5 antibody, Calpain 5, calpain htra-3 antibody, Calpain 5, calpain 5 antibody, HTRA3 antibody
Cross Reactivity Human
Applications ICC, IF, WB
Immunogen Calpain 5 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 381 of Calpain-5

Images

Immunofluorescent staining using Calpain 5 antibody (70R-12717)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calpain-5 antibody at 1:100 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Calpain 5 antibody was purified by antigen-affinity chromatography
Molecular Weight 73 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.47 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Calpain 5 antibody (70R-12717)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calpain-5 antibody at 1:100 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using Calpain 5 antibody (70R-12717)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 7.5 % SDS PAGE gel and Calpain 5 antibody at a dilution of 1:1000

Availability: In stock

Price: €395.09
Size: 100 ul
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