Calsequestrin2 protein (His tag) (80R-1273)
Purified recombinant Human Calsequestrin2 protein
|Synonyms||CASQ2 protein, Calsequestrin -2, Calsequestrin 2 (cardiac muscle) protein, Calsequestrin 2, Calsequestrin -2 protein, PDIB2 protein, Calsequestrin 2, FLJ93514 protein, Calsequestrin 2 fast twitch cardiac muscle protein, Calsequestrin 2 protein, Cardiac calsequestrin 2 protein, Calsequestrin cardiac muscle isoform protein, PDIB2., Calsequestrin fast twitch cardiac muscle protein, CASQ 2 protein, ESTM52 protein, FLJ26321 protein, Calsequestrin2 protein|
Coomassie Blue stained SDS-PAGE of Calsequestrin2 protein (His tag) (80R-1273)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||20-399 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMEEG LNFPTYDGKD RVVSLSEKNF KQVLKKYDLL CLYYHEPVSS DKVTQKQFQL KEIVLELVAQ VLEHKAIGFV MVDAKKEAKL AKKLGFDEEG SLYILKGDRT IEFDGEFAAD VLVEFLLDLI EDPVEIISSK LEVQAFERIE DYIKLIGFFK SEDSEYYKAF EEAAEHFQPY IKFFATFDKG VAKKLSLKMN EVDFYEPFMD EPIAIPNKPY TEEELVEFVK EHQRPTLRRL RPEEMFETWE DDLNGIHIVA FAEKSDPDGY EFLEILKQVA RDNTDNPDLS ILWIDPDDFP LLVAYWEKTF KIDLFRPQIG VVNVTDADSV WMEIPDDDDL PTAEELEDWI EDVLSGKINT EDDDEDDDDD DNSDEEDNDD SDDDDDE|
|Grade & Purity||> 95% pure|
|Molecular Weight||48.4 kDa (417aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Calsequestrin 2 is a calcium-binding protein of the sarcoplasmic reticulum. The protein helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. It also helps sarcoplasmic reticulum store an amazing amount of calcium ions. Recombinant Calsequestrin 2 protein was expressed in E. coli and purified by using conventional chromatography techniques.|
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