Carbonic anhydrase 1 protein (His tag) (80R-1221)

Purified recombinant Human Carbonic anhydrase 1 protein

Synonyms CAI protein, Carbonic anhydrase B protein, CA1 protein, Carbonic anhydrase 1, Carbonic anhydrase -1 protein, Carbonic anhydrase -1, CA 1 protein, Car1 protein, yadF protein, Carbonic anhydrase 1 protein, Car 1 protein, Carbonic dehydratase protein, CA I protein, CAN protein, Carbonate dehydratase I protein, CAB protein, , ECK0125 protein, Carbonic anhydrase 1 protein, JW0122 protein, CA-I protein, Carbonic anhydrase 1
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Carbonic anhydrase 1 protein (His tag) (80R-1221)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-261 amino acids: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 31.0 kDa (281aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Carbonic anhydrase 1 (CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2. This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of Carbonic anhydrase 1 protein (His tag) (80R-1221) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €206.99
Size: 100 ug
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