Carbonic anhydrase 1 protein (His tag) (80R-1221)
Purified recombinant Human Carbonic anhydrase 1 protein
|Synonyms||CAI protein, Carbonic anhydrase B protein, CA1 protein, Carbonic anhydrase 1, Carbonic anhydrase -1 protein, Carbonic anhydrase -1, CA 1 protein, Car1 protein, yadF protein, Carbonic anhydrase 1 protein, Car 1 protein, Carbonic dehydratase protein, CA I protein, CAN protein, Carbonate dehydratase I protein, CAB protein, , ECK0125 protein, Carbonic anhydrase 1 protein, JW0122 protein, CA-I protein, Carbonic anhydrase 1|
Coomassie Blue stained SDS-PAGE of Carbonic anhydrase 1 protein (His tag) (80R-1221)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-261 amino acids: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F|
|Grade & Purity||> 95% pure|
|Molecular Weight||31.0 kDa (281aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Carbonic anhydrase 1 (CA1) is a zinc metalloenzyme that catalyses reversible hydration of CO2. This protein is fundamental to many biological processes such as cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, salvia, and gastric acid. It is most abundant in erythrocytes and is a very early marker for erythroid differentiation. Recombinant human Carbonic anhydrase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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