Carbonic anhydrase protein (80R-4387)

Purified Recombinant Carbonic anhydrase protein (His tagged)

Synonyms Carbonate dehydratase protein, CAN protein, yadF protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis using Carbonic anhydrase protein (His tag) (80R-4387)

Specifications

Residues 1-220 aa: MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 27 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Storage & Safety

Storage Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O <-> HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life.

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Images

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SDS-PAGE analysis using Carbonic anhydrase protein (His tag) (80R-4387)



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Availability: In stock

Price: €102.25
Size: 20 ug
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