The immunoaffinity purified antibody recognizes 45 kDa pro-Caspase-1 and the 20 kDa cleaved product.
Cross Reactivity
Human, Mouse, Rat
Applications
IF, IP, WB
Immunogen
Caspase 1 antibody was raised in Rabbit using synthetic peptide surrounding amino acid 132 of human caspase-1 in Accession NP_150636 as the immunogen.
Assay Information
Caspase 1 Blocking Peptide, catalog no. 33R-10437, is also available for use as a blocking control in assays to test for specificity of this Caspase 1 antibody
Store at -20 deg C. For long-term storage, aliquot and freeze at -70 deg C. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase 1 (ICE, interleukin 1 converting enzyme) is the mammalian caspase responsible for the proteolytic conversion of the proforms of interleukin 1 and IL 18 into active cytokines. Like other caspases, Caspase 1 itself is synthesized as a pro enzyme that is cleaved during activation into a large (20 kDa) and small (10 kDa) subunits. The cellular role of Caspase 1 appears limited to cytokine activation as they do not have obvious roles in apoptosis.