Caspase 2 Inhibitor Drug Screening Kit (55R-1329)
Screening Kit for detection of Caspase 2 Inhibitor activity in the research laboratory
Overview
Overview
| Synonyms | Capase 2 Inhibitor Kit, Capase 2 Screening Kit, Capase Inhibitor Screening Kit, Capase 2 Inhibitor Assay Kit |
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| Applications | ELISA, FC, Functional Assay, IF |
Specifications
| Form & Buffer | Assay Kit containing the folllowing: 2X Reaction Buffer, Caspase Substrate VDVAD-AFC (1 mM), DTT (1 M), Active Caspase-2 (Lyophilized), Caspase Inhibitor, Z-VAD-FMK (2 mM) |
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Storage & Safety
| Storage | Store at -20 deg C |
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General Information
| Biological Significance | Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase 2 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VDVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active Caspase 2 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of Caspase 2 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. |
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