Caspase 4 antibody (70R-12500)

Affinity purified Rabbit polyclonal Caspase 4 antibody

Synonyms Polyclonal Caspase 4 antibody, Anti-Caspase 4 antibody, Caspase -4 antibody, caspase 4 apoptosis-related cysteine peptidase antibody, protease TX antibody, Caspase 4, caspase-4 antibody, Caspase 4 antibody, ICEREL-II antibody, CASP-4 antibody, Caspase -4, ICE(rel)II antibody, TX antibody, apoptotic cysteine protease Mih1/TX antibody, ICE(rel)-II antibody, CASP4 antibody, caspase 4 apoptosis-related cysteine protease antibody, ICH-2 antibody, Caspase 4, protease ICH-2 antibody, Mih1/TX antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen Caspase 4 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 68 and 162 of Caspase 4

Images

Immunofluorescent staining using Caspase 4 antibody (70R-12500)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using caspase-4 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Caspase 4 antibody was purified by antigen-affinity chromatography
Molecular Weight 43 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, ICC:1:100-1:200, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Caspase 4 antibody (70R-12500)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using caspase-4 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using Caspase 4 antibody (70R-12500)</font></div>     | Immunohistochemical staining of paraffin-embedded SAS xenograft using Caspase 4 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using Caspase 4 antibody (70R-12500)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 10 % SDS PAGE gel and Caspase 4 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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