Caspase 8 antibody (70R-12555)

Affinity purified Rabbit polyclonal Caspase 8 antibody

Synonyms Polyclonal Caspase 8 antibody, Anti-Caspase 8 antibody, FLICE antibody, MACH-alpha-1/2/3 protein antibody, apoptotic cysteine protease antibody, FLJ17672 antibody, MACH-beta-1/2/3/4 protein antibody, MORT1-associated ced-3 homolog antibody, caspase 8 apoptosis-related cysteine peptidase antibody, MGC78473 antibody, FADD-like ICE antibody, FADD-homologous ICE/CED-3-like protease antibody, caspase-8 antibody, Caspase 8, Casp-8 antibody, MACH antibody, Caspase 8 antibody, apoptotic protease Mch-5 antibody, ALPS2B antibody, caspase 8 apoptosis-related cysteine protease antibody, MCH5 antibody, CAP4 antibody, Caspase -8, Caspase -8 antibody, ICE-like apoptotic protease 5 antibody, Caspase 8, CASP8 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen Caspase 8 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 217 of Caspase 8

Images

Immunofluorescent staining using Caspase 8 antibody (70R-12555)

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 8 antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Caspase 8 antibody was purified by antigen-affinity chromatography
Molecular Weight 58 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Caspase 8 antibody (70R-12555)</font></div>     | Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 8 antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using Caspase 8 antibody (70R-12555)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3) using a 7.5 % SDS PAGE gel and Caspase 8 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Immunohistochemical staining using Caspase 8 antibody (70R-12555)</font></div>     | Immunohistochemical staining of paraffin-embedded gastric ca using Caspase 8 antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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