AQP3 antibody (70R-51452)

Purified Polyclonal AQP3 antibody

Synonyms Polyclonal AQP3 antibody, Anti-AQP3 antibody, Aquaporin-3 antibody, AQP-3 antibody, Aquaglyceroporin-3 antibody
Specificity Recognizes endogenous levels of Aquaporin 3 protein
Cross Reactivity Mouse, Rat, Bovine, Monkey, Pig, Sheep
Applications IHC, WB
Immunogen AQP3 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Aquaporin 3 as the immunogen

Images

Western blot analysis using AQP3 antibody (70R-51452)

Western blot analysis of Aquaporin 3 expression in LOVO (A); A431 (B); NIH3T3 (C); rat kidney (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification AQP3 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance AQP3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells.

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Images

  • 

Western blot analysis using AQP3 antibody (70R-51452)



 | 

Western blot analysis of Aquaporin 3 expression in LOVO (A); A431 (B); NIH3T3 (C); rat kidney (D) whole cell lysates.
  • 

Immunohistochemical analysis using AQP3 antibody (70R-51452)



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Immunohistochemical analysis of Aquaporin 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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