HAMA blocking reagent (85R-1001P)

HAMA Blocking Reagent for use in immunoassays such as ELISA

Synonyms Heterophilic Immunoglobulin Blocking Reagent, HA blocking reagent, Heterophilic Immunoglobulin Elimination Reagent, Human Anti-Mouse Antibody Blocking Reagent
Applications ELISA, Immunoassays

Specifications

Form & Buffer Supplied in 10mM PBS, pH 7.5

Storage & Safety

Storage Store at 4 deg C for up to 2 weeks. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Product Use Recommended for application to double-antibody sandwich assay to eliminate the interference caused by different HA, HAMA.
Biological Significance Heterophilic Antibody (HA) interference in double-antibody sandwich reaction models can lead to false positive results due to non specific binding to capture and detection reagents. In particular, non specific binding of Human Anti-Mouse Antibody (HAMA) occurs in approximately 10% of immunodiagnostic assays. Other types of HA include, Heterophilic Antibody Goat Antibody (HAGA), Heterophilic Antibody Sheep Antibody (HASA), and Heterophilic Antibody Rabbit Antibody (HARA). This blocking reagent binds to the interferent HAMAs blocking its reaction with capture antibody or conjugated antibody.

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