Cathepsin B Assay Kit (55R-1320)
Assay Kit for detection of Cathepsin B in the research laboratory
Overview
Overview
| Synonyms | Cathepsin B Activity Assay Kit, Cathepsin activity kit |
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| Applications | Functional Assay |
Specifications
| Form & Buffer | Assay Kit containing the folllowing: CB Cell Lysis Buffer, CB Reaction Buffer, CB Substrate Ac-RR-AFC (10 mM), CB Inhibitor (1 mM) |
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Storage & Safety
| Storage | Store at -20 deg C |
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General Information
| Biological Significance | Apoptosis can be mediated by mechanisms other than the traditional Caspase mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-B Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate RR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. |
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