Cathepsin D antibody (10R-1039)

Mouse monoclonal Cathepsin D antibody

Synonyms Monoclonal Cathepsin D antibody, Anti-Cathepsin D antibody, cathepsin D antibody, CTSD antibody, CLN10 antibody, MGC2311 antibody, neuronal 10 antibody, ceroid-lipofuscinosis antibody, CPSD antibody, cathepsin D (lysosomal aspartyl protease) antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen Cathepsin D antibody was raised in mouse using recombinant human Cathepsin D (21-412 aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of Cathepsin D antibody (10R-1039)

Cell lysates of HepG2, Hep3B, MDA-MB-231 and MCF7 (each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system.

Specifications

Host Mouse
Clone 4G2
Isotype IgG2b kappa
Source Anti-human cathepsin D antibody, clone 4G2, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human cathepsin D protein.
Method of Purification Cathepsin D antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The aspartic protease cathepsin D is one of the major proteolytic enzymes in lysosomes. The cathepsin D gene locates on chromosome 11p15.5 and spans approximately 11 kb with nine exons. Initially synthesized as an inactive precursor of pro-cathepsin D, the enzyme is subsequently converted to its active forms by proteolytic processing. Breast cancer cells, unlike normal cells, secrete high levels of pro-cathepsin D

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Images

  • Western Blot analysis of Cathepsin D antibody (10R-1039) | Cell lysates of HepG2, Hep3B, MDA-MB-231 and MCF7 (each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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