CCL1 protein (His tag) (80R-1705)
Purified recombinant Human CCL1 protein
|Synonyms||CCL 1 protein, CCL 1, SCYA1 protein, SISe protein, P500 protein, TCA3 protein, Chemokine C-C motif ligand 1 protein, CCL1, CCL-1, I-309 protein, CCL-1 protein|
Coomassie Blue stained SDS-PAGE of CCL1 protein (His tag) (80R-1705)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||24-96 amino acids: MGSSHHHHHH SSGLVPRGSH MKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK|
|Grade & Purity||> 85% pure|
|Molecular Weight||10.8 kDa (94aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 2mM DTT, 10% glycerol and 50mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||CCL1 (chemokine C-C motif ligand 1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. Unlike the other members of the C-C family, CCL1 does not induce chemotaxis in natural killer (NK) cells. Recombinant human CCL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.|
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