CCL5 protein (Mouse) (30R-2225)
Purified recombinant Mouse CCL5 protein
Overview
Overview
| Synonyms | CCL 5, CCL-5 protein, CCL5, CCL-5 protein, CCL-5, CCL 5 protein |
|---|---|
| Specificity | Human |
| Species | Mouse |
| Protein Type | Recombinant |
| Applications | Functional Assay |
| Assay Information | This recombinant mouse IL-16 has been tested in bioassays for chemoattractant activity with maximal activity observed between 10-100 ng/ml. |
Images
Functional Assay using CCL5 protein (Mouse) (30R-2225)
Chemotaxis of mCCR5-transfected CHO cells in response to Mouse CCL5 protein.
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Method of Purification | CCL5 protein was purified by affinity chromatography. |
| Molecular Weight | 8 kDa |
| Form & Buffer | Supplied as a sterile liquid with 0.1 M glycine with 1% BSA, pH3.0, 0.22 um filtered. |
Usage & Assay Information
| Bioactivity | The bioactivity of this protein was determined by transmigration assay of human monocytes. Maximum chemotaxis is observed at less than or equal to 100 ng/ml. |
|---|
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Mouse CCL-5, or RANTES, is an 8 kDa monomeric protein that belongs to the CC- family of chemokines. The chemokines in this family share a common g-protein coupled receptor, CCR5, which they bind to through a conserved core region. In humans, CCR5 is also known to be the co-receptor through which the HIV virus enters the cell. This effect is blocked by the binding of RANTES to CCR5, making these proteins potential targets for HIV therapy. RANTES plays an important role in the immune response by initiating the recruitment of leukocytes to the site of inflammation. It is a chemoattractant for T cells, monocytes, and dendritic cells, and may increase the cytotoxicity and promote proliferation of NK cells. It is reported to be produced by fibroblasts, platelets, and T cells. RANTES is also involved in the progression and metastasis of some forms of cancer, and constitutive expression has been demonstrated in some malignant tumors. Human and mouse RANTES share 85% sequence homology and demonstrate cross-species activity. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product